Table 2.
RNA quantity and quality.
| Microarray samples |
|||||
|---|---|---|---|---|---|
| Group | RIN of total RNA extracted from homogenized cortexa | Number of cells captured | Total RNA RQIb | Amount of RNA before/after amplification (μg) | A260/A280 |
| Control | 8.2 | 510 | 2.6 | 0.025/39.50 | 2.67 |
| Control | 7.9 | 530 | N/A | 0.004/69.30 | 2.37 |
| Control | 8.9 | 520 | 3.1 | 0.014/54.30 | 2.44 |
| Control | 8.8 | 500 | 2.8 | 0.004/39.49 | 2.51 |
| Control | 7.8 | 680 | 3.4 | 0.007/50.10 | 2.39 |
| Control | 9 | 600 | 3.7 | 0.009/59.92 | 2.54 |
| Control | 8.4 | 530 | 2.8 | 0.012/39.60 | 2.47 |
| Control | 8 | 520 | 3.6 | 0.007/29.83 | 2.75 |
| Control | 8.8 | 520 | N/A | 0.002/24.52 | 2.70 |
| Mean ± SD | 8.42 ± 0.15 | 545.56 ± 19.30 | 3.14 ± 0.16 | 0.009 ± 0.002/40.73 ± 5.15 | 2.54 ± 0.05 |
| Schizophrenia | 8.9 | 525 | 3.8 | 0.004/76.83 | 2.47 |
| Schizophrenia | 8 | 525 | 4.2 | 0.003/65.10 | 2.43 |
| Schizophrenia | 7.9 | 540 | N/A | 0.002/62.10 | 2.52 |
| Schizophrenia | 8.5 | 540 | 2.8 | 0.004/46.92 | 2.75 |
| Schizophrenia | 9.2 | 530 | N/A | 0.001/62.10 | 2.44 |
| Schizophrenia | 8.5 | 630 | N/A | 0.002/75.90 | 2.34 |
| Schizophrenia | 8.2 | 540 | 3.5 | 0.003/65.68 | 2.50 |
| Schizophrenia | 8.8 | 700 | 3.4 | 0.003/50.10 | 2.47 |
| Schizophrenia | 8.4 | 550 | 3.4 | 0.003/52.20 | 2.47 |
| Mean ± SD | 8.48 ± 0.16 | 564.56 ± 20.06 | 3.52 ± 0.19 | 0.002 ± 0.0003/61.88 ± 3.54 | 2.49 ± 0.04 |
RNA integrity number (RIN) of total RNA extracted from homogenized gray matter determined by an Agilent bioanalyzer.
RNA Quality Indication (RQI) of total RNA extracted from laser-captured neurons determined by a BioRad Experion Automated Electrophoresis System. N/A = not available: total RNA concentration too low to determine RQI.