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. 2014 Apr 10;28(1-2):53–69. doi: 10.3109/01677063.2014.882918

Table 2.

RNA quantity and quality.

Microarray samples
Group RIN of total RNA extracted from homogenized cortexa Number of cells captured Total RNA RQIb Amount of RNA before/after amplification (μg) A260/A280
Control 8.2 510 2.6 0.025/39.50 2.67
Control 7.9 530 N/A 0.004/69.30 2.37
Control 8.9 520 3.1 0.014/54.30 2.44
Control 8.8 500 2.8 0.004/39.49 2.51
Control 7.8 680 3.4 0.007/50.10 2.39
Control 9 600 3.7 0.009/59.92 2.54
Control 8.4 530 2.8 0.012/39.60 2.47
Control 8 520 3.6 0.007/29.83 2.75
Control 8.8 520 N/A 0.002/24.52 2.70
Mean ± SD 8.42 ± 0.15 545.56 ± 19.30 3.14 ± 0.16 0.009 ± 0.002/40.73 ± 5.15 2.54 ± 0.05
Schizophrenia 8.9 525 3.8 0.004/76.83 2.47
Schizophrenia 8 525 4.2 0.003/65.10 2.43
Schizophrenia 7.9 540 N/A 0.002/62.10 2.52
Schizophrenia 8.5 540 2.8 0.004/46.92 2.75
Schizophrenia 9.2 530 N/A 0.001/62.10 2.44
Schizophrenia 8.5 630 N/A 0.002/75.90 2.34
Schizophrenia 8.2 540 3.5 0.003/65.68 2.50
Schizophrenia 8.8 700 3.4 0.003/50.10 2.47
Schizophrenia 8.4 550 3.4 0.003/52.20 2.47
Mean ± SD 8.48 ± 0.16 564.56 ± 20.06 3.52 ± 0.19 0.002 ± 0.0003/61.88 ± 3.54 2.49 ± 0.04
a

RNA integrity number (RIN) of total RNA extracted from homogenized gray matter determined by an Agilent bioanalyzer.

b

RNA Quality Indication (RQI) of total RNA extracted from laser-captured neurons determined by a BioRad Experion Automated Electrophoresis System. N/A = not available: total RNA concentration too low to determine RQI.