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Verified full-length gene sequence of BDNF cDNA. A 750 bp PCR product for cDNA BDNF was present on a 1% agarose gel (A). The full-length cDNA BDNF was then cloned into a pZsYellow1-N1 Vector and verified by sequencing (B), as well by immunofluorescent detection (anti-BDNF antibody from Abcam ab620; 1:150) in hippocampal (C) and pituitary gland (D) primary cell cultures of adult CD1 mice.
