FIGURE 7.

The reprogrammed cells were directly imaged to observe the mOrange, which was linked to the 20866 vector, and ZsYellow1, which was linked to the cDNA BDNF. Both fluorescent channels showed positive signals for the reprogramming vector (mOrange) and BDNF (ZsYellow) and these were highly co-localized (merge). Mice that received the non-reprogrammed control cells failed to display and positive cellular fluorescent staining and are hence, not shown. However, the upper (A) and lower (B) photomicrographs depict images of positive soma and fiber projections, respectively, from the vector 20866 + BDNF cDNA infused animals. Importantly, the specific genes were within the same reading frame and images were collected immediately adjacent to the site of infusion.