Fig. 2.
Cellular respiration and changes in the mitochondrial cytochrome redox states monitored by VLS. RAW 246.7 cells were placed in a closed respiration chamber and allowed to consume O2 until anoxia. (A) On-line recording of changes in [O2] (solid black line) and VO2 (dotted gray line) against time using the VLS system. (B) On-line recording of changes in concentration of cytochromes bH (filled circles), cc1 (filled triangles), and aa3 (open squares) from the steady-state against time. Negative values indicate an increase in reduced and a decrease in oxidized cytochromes and vice versa. (C) Changes in VO2 (dotted gray line), normalized between 1 and 0, and Y, the normalized changes in cytochrome redox states for cytochromes bH (filled circles), cc1 (filled triangles), and aa3 (open squares), against [O2].