Fig. 4.

Effect of endogenous NO on the production of and nuclear migration of NF-κB at different [O₂]. (A) Fluorescence, as measured by flow cytometry from intact cells incubated with DHE for 30 min, indicating intracellular production in the presence (shaded) or absence (clear) of l-NMMA at 21% O₂. (B) Fluorescence, as described in A, indicating intracellular production in the presence (shaded) or absence (clear) of l-NMMA at 3% O₂ (C) Western blot of nuclear extracts of cells incubated at 21 and 3% O₂ detecting NF-κB (p65 subunit) in the absence (-) or presence (+) of l-NMMA at 30 and 60 min.