Figure 8.

Lifespan of the zero Ty1 copy strain increases to that of high Ty1 copy strains in hydroxyurea with antioxidant treatment. (A) Median, “50% viability,” and maximum, “10% viability,” lifespans for zero Ty1 copy and high Ty1 copy strains (blue and orange columns, respectively) grown in YPD medium with 30 mM hydroxyurea and the indicated concentration of glutathione, “5 mM GSH” or “10 mM GSH.” Data represent means ± SDs of three to five trials. (B) Mean of normalized dihydrorhodamine (DHR), fluorescence obtained from at least three trials of zero Ty1 copy and high Ty1 copy (blue and orange columns, respectively) strains after the indicated number of days in rich medium with 30 mM hydroxyurea and the indicated concentration of glutathione, “5 mM GSH” or “10 mM GSH.” Error bars indicate standard deviation.