Figure 8.

Mutations that prevent binding of Ert1 to the PDC1 promoter result in increased activity of a PDC1-lacZ reporter. (A) Schematic view of the PDC1-lacZ reporter. The grey rectangle corresponds to the UAS (upstream activating sequence) present in the PDC1 promoter. The DNA sequence of the region bound by Ert1 is shown below. The key nucleotides for Ert1 binding are boxed and the CGG triplets (opposite strand) necessary for Ert1 binding are in boldface type. Mutations that were introduced in the reporter are underlined and in boldface type. (B) Strain YPH499 containing a wild-type PDC1-lacZ reporter (or mutants) integrated at the URA3 locus was grown in the presence of 2% glucose or 2% ethanol and β-galactosidase activity was measured (see Materials and Methods for more details). Results are the average of two experiments performed in duplicate and with two independent strains. In addition to histograms, one numerical value is given when a low β-galactosidase activity was measured. WT, wild\x{2010}type.