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. 2014 Aug 21;198(2):561–575. doi: 10.1534/genetics.114.169409

Figure 3.

Figure 3

puf-8; lip-1 increased mRNAs are enriched for oogenic mRNAs. (A) Tissue expression of mRNAs differentially expressed between reprogramming competent puf-8; lip-1 and noncompetent puf-8 fbf-1; lip-1 animals. The publicly available Nematode Expression Pattern DataBase in situ hybridization database (NEXTDB: nematode.lab.nig.ac.jp/) contained hybridizations for 82 of the 219 mRNAs differentially expressed between reprogramming competent and noncompetent animals; these mRNAs were scored for expression in germline, intestine, and other somatic tissues (pharynx, hypodermis, body wall muscle, vulva, and neuron). (B) Sex-specific germline expression of mRNAs differentially expressed between reprogramming competent and noncompetent animals. The 63 germline-expressed mRNAs from A were first separated as being either at higher levels in the reprogramming competent animals (“RC-increased”) or at lower levels in the reprogramming competent animals (“RC-reduced”); mRNAs in these two groups were then scored for expression during oogenesis (pink), spermatogenesis (blue), or both (gender neutral, in black). (C–F) mRNAs in distinct data sets were compared to RC-increased (red), RC-reduced (blue), and the genome (gray). (C) Maternally expressed mRNAs (Baugh et al. 2003). (D) Oogenesis mRNAs (Reinke et al. 2004). (E) EFL-1 targets (Gerstein et al. 2010). (F) GLD-1 targets (Jungkamp et al. 2011). n.s., not significant; **FDR < 1E-6; ***FDR < 1E-9.