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. 2014 Jul 31;198(2):605–616. doi: 10.1534/genetics.114.166538

Figure 2.

Figure 2

Western blots of Sup35NMspecies+C. Cells of indicated genotype were grown on dextrose or galactose, and extracts made and Western blots prepared as described in Methods. “x” is the species that is the source of NM. cer = S. cerevisiae, mal = C. maltosa, lac = K. lactis, gos = A. gossypii, pom = S. pombe, nid = A. nidulans, fum = A. fumigatus, gri = M. grisea, may = U. maydis, neo = C. neoformans. (1) Dextrose YHE1223 with pCEN LEU2 PSUP35 SUP35NMx+C. (1a) Dextrose YHE1223 with pCEN LEU2 PSUP35 SUP35NMx+C and 2μ pTRP1 PGAL1 SUP35NMx+C. (1b) Galactose YHE1223 with pCEN LEU2 PSUP35 SUP35NMx+C and 2μ TRP1 PGAL1 SUP35NMx+C. (2) Dextrose YHE1223 SUP35NMx+C integrated. (3) Dextrose YHE1223 SUP35NMx+C integrated and 2μ LEU2 PGAL1 SUP35NMx+C. (4) Galactose YHE1223 SUP35NMx+C integrated and 2μ LEU2 PGAL1 SUP35NMx+C.