Table 2. Overproduction of Sup35NMspeciesGFP measured by fluorescence of whole cells.

Plasmid	NM–GFP	Fold expression
pH317	Vector	1
pAZ018	GFP	41
pAZ017	S. cerevisiae	16
pAZ15	K. lactis	33
pH1301	C. albicans1	1
pH1302	C. albicans2	7
pH1303	U. maydis	33
pH1304	A. fumigatus	68
pH1305	C. neoformans	22
pH1306	D. hansenii	18
pH1307	N. crassa	39
pH1308	C. glabrata	16
pH1309	C. maltosa	2
pH1310	A. gossypii	40
pH1311	S. pombe	45
pH1312	A. nidulans	50
pH1313	M. grisiae	64

Plasmids used to overexpress Sup35NMspecies for prion induction were modified to encode GFP at the C terminus of the Sup35NM. Strain HJK088 carrying these plasmids was grown under the same conditions used to induce [PSI+] in the same strain. Fold expression was measured by GFP fluorescence (see Materials and Methods) compared to cells carrying the vector alone. Expression of Sup35NMs that did not produce [PSI+] was at least as good as those that did.