Table 3. [PSI+] generation by SUP35NM homologs integrated at the genomic SUP35 locus.

Source of SUP35NM	Strain	Inducing plasmid		Ade+/106 cells	n	Cytoduct. Recipient	Total Ade+ isolates tested	Isolates giving Ade+ cytoductants
S. cerevisiae	HJK088	pH317	vector	31	8	YAZ013	44	0
		pHK006	NM cer	23,900	8		47	45
		pH1294	NM cer + C	82,900	8		47	45
C. albicans-1	HJK089	pH317	vector	143	8	YHE1387	144	0
		pHk006	NM cer	162	8		144	0
		pHK007	NM alb1	1,850	8		144	12
		pH1295	NM alb1 + C	1,470	8		144	3
K. lactis	HJK092	pH317	vector	79	8	YHE1425	144	0
		pHK006	NM cer	136	8		144	0
		pHK016	NM lac	169	8		144	0
		pH1296	NM lac + C	637	8		144	7
C. maltosa	HJK122	pH317	vector	13	4	YHE1389	48	0
		PHK006	NM cer	23	4		48	0
		pHK012	NM mal	32	4		48	0
		pH1299	NM mal + C	24	4		95	0
A. gossypii	HJK111	pH317	vector	77	4	YHE1391	48	0
		pHK006	NM cer	115	4		48	0
		pHK018	NM gos	129	4		48	0
		pH1300	NM gos + C	84	4		48	0
S. pombe	HJK110	pH317	vector	24	3	YHE1383	48	0
		pHK006	NM cer	41	4		47	0
		pHK010	NM pom	24	4		48	0
		pH1297	NM pom + C	12	4		48	0

The indicated SUP35NMspecies+C fusion genes were integrated into strain YHE1223 replacing the sup35::kanMX marker, and pJ533 (CEN URA3 SUP35cer) was eliminated. SUP35 homologs not listed could not be integrated into the genome of this strain. In general these are the same SUP35 homologs that showed poor suppression of ade2-1. An inducing plasmid with SUP35NMcer (pH952), SUP35NMspecies, or SUP35NMspecies+C (or just the vector), each with the GAL1 promoter, was introduced and cells were grown in galactose to induce prion formation by overproduction of the fusion protein or NM. Cells were plated on –Ade, clones were counted at 6 days, and the results shown are the average of n experiments. Cytoduction recipients have the same SUP35 integrated at the genomic SUP35 locus. The cytoduction recipients are not isogenic as they were created by meiotic crosses. The “total” number of Ade+ isolates were used as cytoduction donors.