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Snf1 kinase is required for resistance to 2DG on glucose. 2DG resistance assays were conducted comparing wild-type cells to snf1∆ cells (A); to cells expressing one or none of the Snf1 β subunits, Gal83, Sip1, or Sip2 (B); to cells lacking one or all of the Snf1 activating kinases, Sak1, Tos3, or Elm1 (C); or to cells expressing Snf1 kinase with single-amino-acid substitutions (D).
