Figure 7.

Persistent inhibition by 2DG. (A) Four pathways of glucose metabolism in yeast are shown. 2-Deoxyglucose can be converted to 2-deoxyglucose-6-phosphate by hexokinase II (Hxk2) and used for cell wall synthesis and storage carbohydrate metabolism. 2-Deoxyglucose-6-phosphate cannot proceed further in glycolysis than the step catalyzed by phosphoglucose isomerase (Pgi1) but could enter the pentose phosphate pathway through oxidation by glucose-6-phosphate dehydrogenase (Zwf1). Further metabolism in the pentose phosphate pathway is blocked at the step catalyzed by 6-phosphogluconate dehydrogenase (Gnd1). (B) 2DG resistance assay of wild-type cells compared to zwf1∆ and hxk2∆ cells. (C) Wild-type cells were grown in duplicate cultures in YEPD media overnight in the absence (circles) or presence (triangles) of 0.2% 2DG. Cells were harvested, washed in YEPD, and diluted to an A₆₀₀ of 0.15 in YEPD media. Cell growth was measured by reading absorbance at 600 nm. Lag times and doubling times for the cultures with and without 2DG treatment are indicated.