Fig. 2.

HIV-1 infection does not mature DCs. (a) iDC and DCs matured with a cytokine mixture (mDC) were infected with BaL and stained for the DC–LAMP maturation marker and p24 (intracellular staining). Similar findings were made in eight experiments, including other maturation stimuli like LPS and CD40 ligation (Table 2). (b) The iDCs and mDCs were infected with Yu2-HIV-1/GFP and analyzed for maturation markers (y axis) by FACS after 5 days (surface staining). (c) Allo MLR stimulation was monitored by CFSE dilution by using CFSE-labeled T cells and the indicated DCs. The experiment shown is representative of three or more experiments.