Fig. 5.

Cocultures of infected immature DCs and T cells inhibit a MLR. DCs from HIV-1-infected patients were infected or not with HIV-1 for 4 days and then added to autologous T cells for 7 days. The indicated cells (Center) were then placed in a transwell chamber. In the lower wells, an MLR was generated, and proliferation was assessed by CFSE dilution. T CFSE indicates T cells cultured without DCs. (A)(Left) Correlation between suppression of the MLR and production of IL-10 (Table 2). (Right) Comparison between immature and mature DCs to show the suppression by iDC HIV + T (fourth row). (b and c) Comparison between iDCs infected with HIV-1 or pulsed with gag protein (b) or peptides (c). (d) Neutralizing anti-IL-10 or isotype control antibody was added at 10 μg/ml, and healthy controls studied in parallel (Left).