Table 2.

Real-time PCR primer sequences of a panel of genes involved in inflammatory response and housekeeping genes

Target genes	Primer sequences	GenBank accession #
IL-1b	Forward 5′-GGACCCCAAAAGATGAAGGGCTGC-3′	NM_008361
	Reverse 5′-GCTCTTGTTGATGTGCTGCTGCG-3′
IL-6	Forward 5′-CCTCTCTGCAAGAGACTTCC-3′	NM_031168
	Reverse 5′-CTCCGGACTTGTGAAGTAGG-3′
IL-12b	Forward 5′-AACCAGAAAGGTGCGTTCCTC-3′	NM_008352
	Reverse 5′-ATGCCCACTTGCTGCATGA-3′
TNF-α	Forward 5′-ATGGCCCAGACCCTCACACTCA-3′	NM_013693
	Reverse 5′-TGGTGGTTTGCTACGACGTGGG-3′
IL-10	Forward 5′-GCGGCTGAGGCGCTGTCAT-3′	NM_010548
	Reverse 5′-GGCCTTGTAGACACCTTGGTCTTGG-3′
Ccl2	Forward 5′-AGG TGT CCC AAA GAA GCT GTA-3′	NM_011333
	Reverse5′-TCT GGA CCC ATT CCT TCT TG-3′
Ccl4	Forward 5′-AGCCAGCTGTGGTATTCCTGACCA-3′	NM_013652
	Reverse 5′-TCATGTACTCAGTGACCCAGGGCT-3′
Cxcl1	Forward 5′-CCTGCAGACCATGGCTGGGAT-3′	NM_008176
	Reverse 5′-GTGTGGCTATGACTTCGGTTTGGG-3′
Cxcl2	Forward 5′-GTTTGCCTTGACCCTGAAGCCCC-3′	NM_009140
	Reverse 5′-CCAGGTCAGTTAGCCTTGCCTTTGT-3′
Csf2	Forward 5′-TCGAGCAGGGTCTACGGGGC-3′	NM_009969
	Reverse 5′-GTCCGTTTCCGGAGTTGGGGG-3′
Hprt	Forward 5′-CAGTCCCAGCGTCGTGAT-3′	NM_013556.2
	Reverse 5′-CAAGTCTTTCAGTCCTGTCCATAA-3′
Ppia	Forward 5′-CAC CGT GTT CTT CGA CAT CA-3′	NM_008907.1
	Reverse 5′-CCA GTG CTC AGA GCT CGA AAG-3′
Tbp	Forward 5′-TCCCAAGCGATTTGCTGCAGTCATC-3′	NM_013684
	Reverse 5′-ACTCTTGGCTCCTGTGCACACCA-3′