Table 1.
SOS regulated genes
| LexA-dependent genes | Function | Identification as LexA dependent | LexA Box(es)a |
|---|---|---|---|
| polB (dinA) | −2 Frameshifts and replication restart after UV treatment (29, 126, 304, 305) |
Mud1(Ap lac) (182) |
−71 (75, 113) |
| dinB (dinP) | Translesion synthesis of N2-dG adducts and −1 frameshifts (169, 188) |
Mud1(Ap lac) (182) |
−32 (75, 113) |
|
hokE (ybdY) |
Killing protein (113) | Northern Analysis (113) | −97 (75, 113) |
| uvrB | Involved in nucleotide excision repair (159) | UV resistance during Hfr mating; Mud1 (Ap lac) (115, 159, 182) |
−92 (75, 113) |
| dinG | 5´–3´ ssDNA helicase (195, 385, 387) | Galactokinase gene fusion (207) | −105/−34 (75, 113) |
| ftsK (dinH) | Chromosome segregation at cell division plane (96) |
Galactokinase gene fusion (207) | −96 (75, 113, 207) |
| sulA | Inhibitor of cell division (132) | SOS chromtest; Mud1(Ap lac) (160, 295) | −42 (75, 113) |
| dinI | Stabilizes RecA/ssDNA filaments (386, 413) | Mud1(Ap lac); Suppressor dinD68; lacZ fusion (182, 413, 415) |
−37 (75, 113) |
| umuCD | DNA pol V involved in SOS mutagenesis and translesion DNA synthesis (266, 286, 354) |
LexA inhibition of in vitro transcription; MudI (17, 190) |
−331,−351/−57,−37 (75, 113, 190) |
| yebG | Unknown | Operon Fusion (225) | −35 (75, 113, 225) |
| ruvAB | Holliday junction branch migration (RuvA DNA binding/RuvB helicase motor) (370, 371) | LexA repression of in vitro RNA pol. Assay; LacZ fusion (343, 344) |
−67 (75, 113) |
| sbmC | DNA gyrase inhibitor (19, 63, 264) | Microcin resistance (19) | −32 (75, 113) |
| ssb | Single stranded binding protein (6, 245, 346) | S1 mapping; Galactokinase gene fusion (44) |
−170 (113) |
| molR (dinOsosF) | Molybdate transport (205) | Northern Analysis (113) | −27 (75, 113) |
| recN | Involved in recombinational repair (222, 242, 290, 330, 396) | 2D–gel electrophoresis; Mu (Ap lac) fusion (114, 222, 291) |
−28, −46, −66 (75, 113) |
| recA | SOS regulation and mutagenesis/homologous recombination (36, 52, 77, 109, 148, 157, 198, 211, 213, 216, 218) |
Dnase I protection assay; LacZ fusion (60, 218) |
−77 (75, 113) |
| pcsA (dinD) | Unknown | Mud1(Ap lac) (182) | −61 (75, 113) |
| uvrD | DNA helicase II (272) | Complementation UVs and MMSs (234) |
−74 (75, 113) |
| dinF | Unknown down stream of LexA | Mud1(Ap lac) (182) | −24,−45 (75, 113) |
| LexA | Transcriptional repressor of SOS genes (45, 46, 128, 157, 214, 218) | Dnase I protection assay (218) | −24,−45 (75, 113) |
| uvrA (dinE) | Involved in nucleotide excision repair (159) | UV resistance during Hfr mating; Mud1 (Ap lac) (159, 183) |
−101/−168 (75, 113) |
| dinQ | Unknown | Northern Analysis (113) | −139 (113) |
| ydjQ | Putative UvrC homolog, function unknown (254) |
Northern Analysis (113) | −33 (75, 113) |
| ysdAB (tisAB) | Toxic protein expressed under stress conditions (384) |
Northern Analysis (113) | −142 (113) |
| ydjM | Inner membrane protein (83) | Northern Analysis (113) | −52 (113) |
| dinS | Putative transposase (113) | Northern Analysis (113) | −74 (113) |
| yigN | Unknown | Microarray (75) | −55 (75, 113) |
| ybfE | Unknown | Northern Analysis (113) | −136 (113) |
| yjiW (dinLsosC) | Unkown | Northern Analysis (113) | −95 (75, 113) |
| borD | Homolog of prophage protein expressed during lysogeny (21, 22) |
Microarray (75) | ND |
| ybiN | Unknown | Microarray (75) | ND |
| grxA | Glutoredoxin; Hydrogen donor for ribonucleotide reductase (293) |
Microarray (75) | ND |
| yccF | Unknown | Microarray (75) | ND |
| ymfD | Unknown | Microarray (75) | ND |
| ymfE | Unknown | Microarray (75) | −280 |
| Lit | Protease for EF-Tu (134) | Microarray (75) | −193 |
| intE | Predicted phage integrase | Microarray (75) | 84, −192, −195 (75) |
| ymfG | Unknown | Microarray (75) | ND |
| ymfH | Unknown | Microarray (75) | ND |
| ymfI | Unknown | Microarray (75) | 84 |
| ymfJ | Unknown | Microarray (75) | ND |
| ycgH | Unknown | Microarray (75) | ND |
| ydeO | Unknown | Microarray (75) | −272 |
| ydeS | Unknown | Microarray (75) | −43 |
| ydeT | Unknown | Microarray (75) | ND |
| ydeR | Unknown | Microarray (75) | ND |
| arpB | Unknown | Microarray (75) | ND |
| yoaB | Unknown | Microarray (75) | −123 |
| ogrK | Regulate gene transcription of phage P2 (145, 410) | Microarray (75) | −193, 8 |
| yqgC | Unknown | Microarray (75) | −41, −193 |
| yqgD | Unknown | Microarray (75) | ND |
| yhiJ | Unknown | Microarray (75) | ND |
| yhiL | Unknown | Microarray (75) | −63, −187 |
| glvB | Unknown | Microarray (75) | −174 |
| ibpB | Heat inducible chaperone (199) | Microarray (75) | ND |
| ibpA | Heat inducible chaperone (199) | Microarray (75) | −249 |
| yifL | Unknown | Microarray (75) | ND |
| LexA-independent genes | Function | Identification as SOS induced | |
| dinY | Mutant defective in Weigle reactivation of UV-irradiated bacteriophage λ (288) | Mu d1(Ap lac) (288) | |
| dnaA | Initiation protein for chromosomal replication (399) |
Transcriptional fusions; S1 nuclease mapping, immunoblot analysis (297) |
|
| dnaB | Replicative DNA helicase (204) | lacZ fusion (191) | |
| dnaN | Pol III processivity factor; β-subunit (171) | lacZ fusion (171) | |
| dnaQ | Pol III proofreading factor; ε-subunit (171) | lacZ fusion (171) | |
| hga | 2-Keto-4-hydroxyglutarate adolase; respiration recovery after UV exposure (106, 281) |
2D–Gel Electrophoresis, Peptide Sequencing, and O2 consumption (62) |
|
| ihfA (himA) | Site-specific recombination (248, 249, 251) |
lacZ fusion; Radiolabled 2D–Gele electrophoresis (250) |
|
| nrdAB | Ribonucleotide reductase (121–125) | lacZ fusion (135) | |
| phr | DNA photolyase (327) | lacZ fusion (163, 283) | |
| hslVU | Protease of SulA (310, 337, 416, 417) | Microarray (75) | |
| cvpA | Colicin production (111) | Microarray (75) | |
| purF | Purine biosynthesis (313) | Microarray (75, 314) | |
| upp | Pyrimidine scavaging (8) | Microarray (75) | |
| rpoD | RNA polymerase sigma factor (263) | Microarray (75) | |
| dnaG | DNA primase (31, 349) | Microarray (31, 75) | |
| rpsU | Ribosomal subunit (82) | Microarray (75) | |
| yfaE | Unknown | Microarray (75) | |
| recX | Inhibitor of RecA (357) | Microarray (75) | |
| recQ | DNA helicase (378) | Suppression dnaE486; in vitro helicase assays (165) | |
| sfiC | Inhibition of cell division (81) | Cell Filamentation in recAsfiA99::Mu d(Ap lac) strain (81) |
|
a
Distance from ATG start codon.