Table 1.
Legend Age-matched male mice 5-10 weeks of age were immunized i.p. with 100 μl of either 5 × 104 PFU of LCMV diluted 70-fold from stock virus into serum-free Hank's Balanced Salt Solution (HBSS), 107 PFU of PV purified in sucrose gradients and diluted in HBSS, or HBSS only (Naïve control). A higher dose of PV was used because this stock of PV does not replicate as well in mice as LCMV. After at least 6 weeks the mice were given heterologous challenges with similar doses of LCMV or PV. Four days after challenge spleens were harvested, and spleen suspensions were analyzed for PFU. Data presented are the means ± standard deviation, with the number of mice per group in parentheses. Experiments were designed with n=4-5 per group. In three experiments (#3, 7, and 12) there was unexpected death of some mice prior to challenge. In this table we decided to present all the data we had rather than to delete those experiments. Replication of Virus in the Spleen of Naïve or Immune Mice
| Expt. | Mouse | (MHC) | Naïve/PV | LCMV-imm/PV | P |
|---|---|---|---|---|---|
| 1. | C57BL/6 | (b) | 2.9±0.2 (5) | 1.4±0.7 (4) | .002 |
| 2. | B10 | (b) | 3.3±0.5 (5 ) | 1.5±0.7 (5) | .002 |
| 3. | B10 | (b) | 3.8±0.3 (2) | 1.9±0.7 (4) | .025 |
| 4. | CBA | (k) | 5.4±.04 (5) | 5.5±0.2 (5) | .30 (NS) |
| 5. | CBA | (k) | 5.8±0.5 (5) | 5.2±0.8 (5) | .60 (NS) |
| 6. | B10.BR | (k) | 3.7±0.6 (4) | 3.2±0.5 (4) | .25 (NS) |
| 7. | B10.BR | (k) | 3.5±0.6 (2) | 3.7±0.5 (4) | .68 (NS) |
| 8. | B10.BR | (k) | 3.8±0.5 (5) | 3.7±0.4 (5) | .35 (NS) |
| 9. | BALB/c | (d) | 5.6±0.4 (4) | 5.1±0.2 (4) | .067 |
| 10. | BALB/c | (d) | 5.6±0.2 (4) | 5.3±0.1 (4) | .036 |
| 11. | B10.D2 | (d) | 3.3±0.6 (5) | 2.9±1.1 (5) | .49 (NS) |
| 12. | B10.D2 | (d) | 4.5±0.5 (2) | 3.4±1.3 (4) | .33 (NS) |
| 13. | B10.D2 | (d) | 4.2±0.5 (5) | 3.1±0.3 (4) | .04 |
| Naïve/LCMV PV-imm/LCMV | |||||
| 14. | BALB/c | (d) | 4.5±0.63 (4) | 3.8±0.3 (4) | .08 |
| 15. | BALB/c | (d) | 5.1±0.02 (4) | 4.0±0.7 (4) | .02 |
| 16. | CBA | (k) | 4.6±.05 (5) | 4.4±.05 (5) | .0002 |