Figure 5. AZD8055 inhibits T cell IFNγ production at day 10 post-transplant.

Splenocytes were restimulated with PMA and ionomycin on day 10–11 post-transplant and intracellular cytokine production assessed by flow cytometry. (A, B) CD8⁺ and (C, D) CD4⁺ T cell production of IFNγ is depicted and quantified. (E) IFNγ production by CD4⁺ and CD8⁺ T cells, and IL-17⁺, Foxp3⁺ and IL-10⁺ CD4⁺ T cells were quantified on days 10, 20 and 80 post-transplant. * p<0.05; *** p<0.001. n=4 mice per group. IS=immunosuppression.