Time course of TMR-Aβ
aggregation in the presence of various
forms of the apoE proteins. Panels A–C show the aggregation
of TMR-Aβ1–40: (A) 4 μM TMR-Aβ1–40 in the presence of 0, 50, 100, and 200 nM recombinant
lipid-free apoE4 and (B and C) 4 μM TMR-Aβ1–40 in the absence or presence of 150 nM recombinant lipid-free apoE
(B) and DMPC–apoE (C), respectively. Panels D–F show
the aggregation of TMR-Aβ1–42: 2 μM
TMR-Aβ1–42 in the absence or presence of recombinant
lipid-free apoE (50 nM), DMPC–apoE (50 nM), and astrocyte-derived
apoE (11 nM) respectively.