A. Left: a normal electrocardiogram or rat before the model building. Right: After the model building, electrocardiograms lead II indicated ST segment elevated significantly, which indicated ischemia myocardial and proved the artery ligation was successful. B. The cells were in the shape of stars or polygons when they initially disassociated out of the tissue block and had a low density, they were in the shape of paving stones when the cells bespreaded the bottom of the culture flask, in some of which tube structure and vessel network structure could be spotted. C. Immunocytochemical stain revealed that: (left) after the staining of VIII factor, the cytoplasm was brown coloring, the coloring was the most significant in pericaryon; (right) after the staining of CD31, their cell membrane showed yellowish brown particles, which proved that the cultured cells were CMECs.