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. 2014 Oct 14;9(10):e108468. doi: 10.1371/journal.pone.0108468

Figure 4.

Figure 4

A. For normal CMECs, observation under inverted phase contrast microscope (100×) revealed that amounts of “C”-shaped tube structure were formed one day after inoculation. The “C”-shaped tube structures were clearer and increased significantly two days after inoculation. The “C”-shaped tube structure decreased with an increased cell number three days after inoculation. The “C”-shaped tube structure further decreased four days after inoculation. For ischemic CMECs, there was no obvious tube formation one day after inoculation, only several “C”-shaped structure were spotted under inverted phase contrast microscope (100×). Several tube formations were spotted two days after inoculation, of which the number was less than that of normal CMECs. The tube structure decreased with an increased cell number three days after inoculation. The tube structure disappeared four days after inoculation. B. A dynamic observation revealed that the migration phase of normal/ischemic CMECs was the second day. Tube formation ratio of ischemic CMECs decreased compared with that of the normal CMECs, but that was of no statistical significance.