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Depletion of γ-CYA but not β-CYA induces actin polymerization. A549 cells transfected with control or cytoplasmic actin isoform–specific siRNAs were subjected to detergent fractionation to determine the G-/F-actin ratio (A, B) or fluorescence labeling with Alexa 555–phalloidin to visualize actin filaments (C). Arrows highlight basal stress fibers in γ-CYA–depleted cells. Data are presented as mean ± SE (n = 3); *p < 0.05.
