FIGURE 6:

EMyT induction in γ-CYA–depleted epithelial cells is controlled by actin polymerization, and γ-CYA preferentially interacts with MRTF. (A, B) γ-CYA–depleted A549 cells were treated for 24 h with either vehicle or the F-actin–depolymerizing drug Lat B (1 μM), and expression of EMyT markers was determined by immunoblotting. Data are presented as mean ± SE (n = 3); *p < 0.05. (C) Association of MRTF-A with γ-CYA and β-CYA in control and γ-CYA–depleted A549 cells was examined using immunoprecipitation with anti–MRTF-A antibody.