FIGURE 3:
Cdc42 knockdown reduces the efflux of serotonin upon exocytosis. BON cells were treated with control siRNAs (blue), siCdc42-A (red), siCdc42-C (green), or siCdc42-A and the rescue-A construct (black) and stimulated with ionomycin. Log-transformed values of the maximal spike height Imax and quantal size Q of amperometric spikes are reduced upon Cdc42 silencing, as indicated by the distribution of pooled spike values (A, B, E, F, I, J) and the mean values of these parameters measured in each cell (C, D, G, H, K, L). The significance of the differences in distributions was computed using an ANOVA test (see Materials and Methods) followed by a Tukey posttest; N = 3610 (control in A and B), 2954 (siCdc42-A in A and B), 1988 (control in E and F), 1586 (siCdc42-C), 2126 (control in I and J), 1334 (siCdc42-A in I and J), and 1642 (rescue-A) spikes. Significance of differences of mean values was computed by Mann–Whitney test (C, D, G, H) or Kruskal–Wallis test followed by Dunn's posttest (K, L).