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. 2014 Oct 15;25(20):3222–3233. doi: 10.1091/mbc.E14-07-1182

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© 2014 Araki et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Transcriptional regulation of STATc target genes and STATc pathway genes. Real-time PCR analysis of selected STATc-dependent genes was carried out with cDNAs from Ax2 wild-type, pyk2−, pyk3−, pyk2−/pyk3−, and STATc− cells either treated for 15 min with 200 mM sorbitol or left untreated. (A) Differential expression of STATc target genes rtoA, sigG , and DDB_G0277667. (B) Differential expression of STATc pathway genes dstc, ptpC, pyk2, and pyk3. The data are expressed as means of fold change in comparison to untreated cells. Fold changes and SEM of three independent experiments with three measurements each. N/A, not applicable. Unpaired t test: *p < 0.05; **p < 0.01; ***p < 0.001; NS, not significant. Statistical significance as compared with expression values in Ax2 cells is indicated.