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. 2014 Oct 14;9(10):e109428. doi: 10.1371/journal.pone.0109428

Figure 5. Xwnt5A induces clustering of ROR2.

Figure 5

A) 150 pg of Xwnt5A-EGFP mRNA were co-injected with 40 pg of the ROR2-mCherry in the two dorsal blastomeres of eight-cell stage embryos. At stage 10.25 dorsal marginal zones were explanted and analyzed for subcellular localization of the Xwnt5A-EGFP and ROR2-mCherry. Prominent Xwnt5A-EGFP clusters co-localize with ROR2-mCherry clusters at the membrane of DMZ cells. These clusters are found at the onset (stage 10.5) and at the end (stage 12) of gastrulation. Shown are snapshots of movies S5 and S6. B) 150 pg of Xwnt5A-EGFP mRNA were co-injected with 1.6 pmol Xwnt-5A specific antisense morpholino and 125 pg Gap43-mCherry. Also in these Xwnt-5A-depleted explants Xwnt5A-EGFP clustered in the membrane.