Figure 5. MiR-615-3p reduces palmitate-induced cell death.
(A) Neonatal liver wild-type cells were transfected with either the negative control precursor or precursor of miR-615-3p, both fluorescently labeled with Cy3. 8 hours after transfection, cells were treated with 400 µM palmitate (PA) or 1 µg/mL tunicamycin for 18 hours. DAPI stained nuclei were counted in each condition. * P<0.05. (B) Neonatal liver wild-type cells were transfected with either the negative control precursor or precursor of miR-615-3p. 8 hours after transfection, cells were treated with 400 µM palmitate (PA) or 1 µg/mL tunicamycin for 18 hours. Caspase 3/7 activity was measured as described. * P<0.05.