Figure 5. Osteoblast differentiation is increased in ERRγ +/− stromal cell cultures.

(A) Bone marrow stromal cells cultured from ERRγ +/− mice had significant increases in CFU-O and CFU-ALP, with no change in CFU-F, compared to cells from ERRγ +/+ mice. Cell counts performed during the proliferation phase revealed no differences between genotypes (N = 8 ERRγ +/+; N = 8 ERRγ +/−). (B) Gene expression analysis of ERRγ +/− versus ERRγ +/+ cultured stromal cells revealed a trend to decreased expression of Bmp, and a significant increase in Alp and Bsp (N = 6 ERRγ +/+; N = 6 ERRγ +/−). All values are expressed as mean ± SD. # = p = 0.055; * = p<0.05; ** = p<0.01; ***p<0.001.