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. 2014 Oct 14;9(10):e110002. doi: 10.1371/journal.pone.0110002

Figure 1. Schematic representation of the experimental design.

Figure 1

At week 0, colitis was induced in immunocompromised SCID mice through i.p. injection of CD4+CD25−CD62L+ T cells; controls were injected with PBS. In the curative treatment protocol (A), mice were treated with SmSWP or vehicle (PBS) at week 4 and week 5 after T cell or PBS injections (i.e., when colitis was established). In the preventive treatment protocol (B), mice were treated weekly for 6 weeks with SmSWP or vehicle (PBS) starting from week 0 (i.e., before colitis was established). During the treatment periods, SCID mice were weighed, a clinical disease score was given and colonoscopy was performed at different time points (weeks 0, 2, 4 and 6). At the end of the study (week 6), mice were sacrificed for postmortem examination of the colon and for examination of the underlying immunological effects of SmSWP. Abbrevations: CD: cluster of differentiation; i.p.: intraperitoneally; PBS: phosphate-buffered saline; SCID: severe combined immunodeficient; SmSWP: Schistosoma mansoni soluble worm proteins.