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. 2014 Oct 14;9(10):e110002. doi: 10.1371/journal.pone.0110002

Figure 2. Multi-color flow cytometric gating strategies for the T cell characterization of the MLN cells.

Figure 2

Gating strategy for the regulatory T cell stain (A): CD4+ cells were initially gated upon expression of CD4 and SSC properties, and subsequently gated and assessed upon expression of CD25 and Foxp3. Gating strategy for the intracellular cytokine stain (B): CD4+ cells were initially gated upon expression of CD4 and side SSC properties, and subsequently gated and assessed upon expression of IFN-γ, IL-17A, IL-4 or IL-10. Abbreviations: CD: cluster of differentiation; Foxp3: forkhead box p3; IFN-γ: interferon-γ; IL: interleukin; MLN: mesenteric lymph nodes; SSC: side scatter.