Table 1. Primers for real-time PCR checking genes related to cytokinin synthesis and flower development.
Gene name | Primer name | Sequence(5′→3′) |
IPT1,4,6 | Fna-75600-F | GAGGAGGCAAGTATGGAAGATAG |
Fna-75600-R | CGACGAACTCGAACTCATCATA | |
IPT2 | Fna-41524-F | CAAACCAGGAGCTGACTATACC |
Fna-41524-R | AGCGGAGCTATTTGTGTCTG | |
IPT3 | Fna-06414-F | TCAGGAATGAGCCGTTCTTAAA |
Fna-06414-R | GTTTGCAAGCTAACCCGAAAG | |
IPT5 | Fna-36855-F | GAGCGGAGAAGCGTGATTAT |
Fna-36855-R | CCGACATGCAAGCAAACAG | |
IPT7 | Fna-44012-F | TTGGGTCGACGTTTCCTTAC |
Fna-44012-R | GCTTTCGGATCGTGTACTTCT | |
IPT8 | Fna-63259-F | GCTTGCCAAGAAGCAGATAGA |
Fna-63259-R | CTCTCTTGACGATGCCCTTAAC | |
IPT9 | Fna-63954-F | GCCGTAGACAAAGAGGTGTAAG |
Fna-63954-R | CATTGAGCCAGTGGTACATAGG | |
CYP735A1 | Fna-68846-F | GAAACTACCGCACTCCTTCTC |
Fna-68846-R | GCAGCCACATACCTCTCTAATC | |
CYP735A2 | Fna-08361-F | CCTCATGCTCCTTGCTCATAA |
Fna-08361-R | TTGCTCAACGGAAGGGATAC | |
URH1 | Fna-72547-F | GGGTGGAGACTCAAGGAATATG |
Fna-72547-R | CATGCCACTGATACTGGTGAA | |
AP1 | Fna-16396-F | TTCTTAGGGCACAGCAAGAG |
Fna-16396-R | GCATGTATGGATGCTGGATTTG | |
AP2 | Fna-27636-F | GCAGATGACGAATTTAACGAAGG |
Fna-27636-R | CTTCCCAACGACCACACTTAT | |
AP3 | Fna-72759-F | ATCGAAGGATCACGTGCTTAC |
Fna-72759-R | AATGATGTCAGAGGCAGATGG | |
PI | Fna-60439-F | AAATGTTGGCGGAGGAGAA |
Fna-60439-R | GAATCGGCTGGACTCTGTATC | |
AG | Fna-37522-F | CTGATGCCAGGAGGAACTAAC |
Fna-37522-R | ATGCCGCGACTTGGAAATA | |
CRC | Fna-16399-F | AAGAGTGCCAATCCGGAAATA |
Fna-16399-R | GCTCCGGAAGTAATGGAAGTAG | |
SPT | Fna-54144-F | CTTTGGACCTTTCCCTCACTT |
Fna-54144-R | CATCAAACGCAGCATGTTCTC | |
LEUNIG | Fna-27110-F | ACAGGCAGTGAAGGAGAATG |
Fna-27110-R | CACCCAATTACGAGTAGGGAAG | |
AINTEGUMENTA | Fna-09982-F | AGACACAGATGGACTGGTAGA |
Fna-09982-R | GAGCAGCTTTCTCCTCCATATC | |
Actin | BnActin 88-F | GCTGACCGTATGAGCAAAGA |
BnActin 88-R | AGATGGATCCTCCAATCCAAAC |