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| antigen | gel band size (kDa)detected | host | supplier | dilution |
| Calcium sensing receptor | 121 | Abcam,Cambridge,USA | 1∶500 | |
| Gelsolin | 90 | Rabbit | 1∶1000 | |
| Cortactin | 80 | Mouse | 1∶2500 | |
| Bone alkaline phosphatase[EPR4477] | 58 | Rabbit | 1∶1000 | |
| BMP-2 | 44 | Rabbit | 1∶1000 | |
| β-actin | 42 | Mouse | 1∶5000 |
