Skip to main content
. 2014 Jun 13;15(7):766–774. doi: 10.15252/embr.201438840

Figure 5. Effect of IGE KCC2 variants on serine 940 (S940) regulatory phosphorylation.

Figure 5

A HEK293 cells were nucleofected with the indicated WT, R952H, and R1049C KCC2 constructs. After 3 days, cells were lysed, and Western blotting was performed using anti-phospho-S940 KCC2 and total anti-KCC2 antibodies, as described in Materials and Methods. A representative Western blot is shown.

B Quantification of total phosphorylation of S940 relative to total KCC2 expression for each construct. 4 separate nucleofections were performed. *P < 0.05, one-way ANOVA test; All pairwise multiple comparison procedures (Tukey’s test). Error bars represent the mean ± s.e.m.