Figure 5. Effect of L-Endoglin overexpression on myofibroblast abundance following ureteral obstruction.

Representative immunohistochemistry images for α-SMA (a) in sham operated (SO), non-obstructed (NO) and obstructed (O) kidneys from WT and L-ENG ⁺ mice. Bar = 100 µm. (b) Quantification of α-SMA-positive stained area in sham operated (SO), non-obstructed (NO) and obstructed (O) kidneys from WT and L-ENG ⁺ mice, expressed as square microns. (c) Western blot analysis of α-SMA protein amount in SO, NO and O kidneys from WT and L-ENG⁺ mice. A representative western blot among 5–7 performed in each group is shown on top. Densitometry analysis is represented as the mean ± SEM of the 5–7 western performed per group. *P<0.01 vs. SO kidneys. #P<0.05 vs. O kidneys from WT mice.