Skip to main content
. 2014 Oct 14;9(10):e110365. doi: 10.1371/journal.pone.0110365

Figure 6. Effect of L-Endoglin overexpression on Smad1 and phospho-Smad1 expression following ureteral obstruction.

Figure 6

(a) Representative immunohistochemistry images for phospho-Smad1 in sham operated (SO), non-obstructed (NO) and obstructed (O) kidneys from WT and L-ENG + mice. Bar = 100 µm. (b) Histogram representing the number of phospho-Smad1-positive nuclei per field in SO, NO and O kidneys from WT and L-ENG + mice. Data is represented as mean ± SEM. Western blot analysis of phospho-Smad1 (c) and total Smad1 (d) protein amount in SO, NO and O kidneys from WT and L-ENG + mice. A representative western blot among 5–7 performed in each group is shown on top. Densitometry analysis is represented as the mean ± SEM of the 5–7 western performed per group. *P<0.01 vs. SO kidneys. #P<0.05 vs. O kidneys from WT mice.