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Lin28a expression levels with representative gel blots of Lin28a and β-actin (loading control) were shown (A); Lin28a mRNA expression as evaluated by real-time PCR analysis (B); Let7a (microRNA) expression as evaluated by real-time PCR analysis (C). Columns and bars represent mean ± SD. Data were obtained from at least three independent experiments. W, wortmannin.*p<0.05 vs. H/R, #p<0.05 vs. H/R+Lin28a.
