Figure 1. Lack of insulin impairs differentiation but not browning capacity of primary pre-adipocytes.

(A) Heatmap showing differential mRNA expression between confluent primary inguinal white adipose tissue (iWAT) precursor cells differentiated for 24 h with white (EtOH treated) or brite (cPGI₂ treated) differentiation cocktail and between absence or presence of insulin (Ins) in the medium. Higher and lower expression is displayed in red and blue, respectively. (n = 3). (B) mRNA expression of UCP-1 and CIDEA or (C) FABP4 and RETN in primary iWAT precursor cells differentiated into white (EtOH treated) or brite (cPGI₂ treated) adipocytes for 8 days with insulin present in the differentiation medium for the indicated timepoints (n = 3). All values in bar graphs are expressed as means ± SEM, #p<0.05, ##p<0.01, ###p<0.001 white (EtOH treated) vs. brite (cPGI₂ treated) cells, *p<0.05, **p<0.01, ***p<0.001 normal conditions vs. insulin deprived conditions.