Fig. 3.

The N-glycan linked to N727 mediates TfR trafficking to ARE in AP-1B KD MDCK cells. (A) Polarized WT (top) and AP-1B KD (bottom) MDCK cells were transiently transfected with either WT or mutant TfR–GFP in each of its four glycosylation sites, and immunostained for Rab11a. (A′) Cells from experiments represented in A were quantified for the percentage of pixels of Rab11a colocalizing with TfR–GFP (left) and the percentage of pixels of TfR–GFP colocalizing with Rab11a (right). Circles correspond to individual cells obtained from different experiments and red lines indicate the mean. ns, not significant; *P<0.05; **P<0.001. Scale bar: 10 µm.