Figure 3. The Dam1 complex loads onto kinetochores in the absence of oligomerization.

(a) Survival versus force curves for beads coated with Ndc80 complex alone (black trace, n = 26) or in the presence of free wild-type (WT, blue trace, n = 60) or oligomerization-deficient (OD, red trace, n = 43) Dam1 complex in solution. (b) As in (a), using beads instead coated with purified kinetochore particles lacking functional endogenous Dam1 complex (dad1-1 kinetochores). Beads were assayed alone (black trace, n = 42), and in the presence of purified, recombinant Dam1^WT (blue trace, n = 81) or Dam1^OD (red trace, n = 78) complex added free in solution.