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. Author manuscript; available in PMC: 2015 Mar 19.
Published in final edited form as: Nat Commun. 2014 Sep 19;5:4951. doi: 10.1038/ncomms5951

Figure 5. Depletion of Hsk3 causes formation of the oligomerization-deficient Dam1 complex in vivo.

Figure 5

(a) Representative images of Dad1-GFP and Ask1-YFP in mitotic control (top), hsk3-degron (middle), and dam1-degron (bottom) cells at 2 hours after addition of auxin. Scale bar is 5 μm and applies to all images. Localization of Dad1-GFP (b) and Ask1-YFP (c) was quantified by automated spot detection and is reported as the average number of detected foci per cell. For both Dad1-GFP and Ask1-YFP, asynchronously growing cells have ~1.4 foci per cell on average, indicating a mix of mitotic (2 foci) and non-mitotic (1 focus) cells. Arrest in mitosis with kinetochore localization causes the average to approach 2.0 foci per cell; delocalization is indicated by a trend towards 0 foci per cell. See Supplemental Figs. 3 and 4 for the number of cells counted in each condition.