Figure 6. Equal bipolar distribution of kinetochores requires oligomerization of the Dam1 complex.

Time-lapse images of spindle poles (Spc110-mCherry, top row), kinetochores (Nuf2-GFP, middle row), and merged channels (bottom row) in control (a) and hsk3-degron (b) cells. Cells were imaged immediately following release from α-factor (G1) arrest; time stamps show time after release. Scale bars are 5 μm. At right in both (a) and (b) are spindle profiles of Nuf2-GFP intensity from corresponding images taken at 135 minutes after release. The integrated intensity in each half-spindle is printed on the plot. (c) Average spindle intensity profiles of Nuf2-GFP in control (black curve) and hsk3-degron (red curve) cells. Markers indicate average ± s.e.m. (d) Box and whisker plots show the distribution of kinetochore intensity ratios measured for control and hsk3-degron cells. Whiskers show the full range of the distribution, while the box shows the 75^th, 50^th, and 25^th percentiles. (e) Plot of kinetochore intensity ratio versus spindle length shows that asymmetric kinetochore alignment in hsk3-degron cells is independent of spindle length. See Methods for additional information about the measurements presented in this figure.