Table 1.
The rupture force assay reveals how oligomerization of the Dam1 complex contributes to microtubule attachment strength.
| Beads functionalized with: | Dam1 complex added free in solution: | Mean RF (pN) | S.E.M. | Median RF (pN) | No. replicates | Compare versus: | P value (KS test) | |
|---|---|---|---|---|---|---|---|---|
| 1 | Dam1WT complex | 6.0 | 0.2 | 5.2 | 120 | |||
| 2 | Dam1OD complex | 4.8 | 0.1 | 4.8 | 69 | 1 | 4.7×10−3 | |
| 3 | Dam1WT complex | 2 nM WT | 7.4 | 0.3 | 6.5 | 106 | 1 | 1.0×10−3 |
| 4 | Dam1WT complex | 2 nM OD | 6.0 | 0.3 | 5.4 | 86 | 1 | 0.97 |
| 5 | Dam1WT complex | 20 nM OD | 6.1 | 0.2 | 5.8 | 89 | 1 | 0.44 |
| 6 | Dam1WT complex | 2 nM WT, 2 nM OD | 5.6 | 0.2 | 5.1 | 109 | 1 | 0.37 |
|
| ||||||||
| 7 | Ndc80 complex | 2.5 | 0.2 | 2.3 | 26 | |||
| 8 | Ndc80 complex | 2 nM WT | 7.4 | 0.4 | 6.9 | 60 | 7 | 1.2×10−12 |
| 9 | Ndc80 complex | 2 nM OD | 4.8 | 0.2 | 4.5 | 43 | 7 | 2.5×10−9 |
|
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| 10 | dad1-1 kinetochores | 2.7 | 0.2 | 2.3 | 42 | |||
| 11 | dad1-1 kinetochores | 100 nM WT | 5.6 | 0.3 | 4.8 | 81 | 10 | 6.8×10−11 |
| 12 | dad1-1 kinetochores | 100 nM OD | 3.7 | 0.2 | 3.5 | 78 | 10 | 2.7×10−5 |