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. Author manuscript; available in PMC: 2015 Oct 8.
Published in final edited form as: Cell Host Microbe. 2014 Oct 8;16(4):531–537. doi: 10.1016/j.chom.2014.09.002

Figure 4. Co-culture of distinct, antimicrobial-resistant SCVs isolated from CF patients enhances growth.

Figure 4

(a) Slowly growing bacterial colonies were isolated and archived from the sputa of adult and pediatric patients suffering from CF at Vanderbilt University Medical Center. Polymicrobial samples were labeled successively as VUCF001, VUCF002, etc. Individual, slowly growing bacterial colonies were isolated from each sample and archived as VUCF001.1, VUCF001.2, etc. Individual small colonies were then co-cultured with ΔhemB or ΔmenE on solid media to assess for enhanced growth. A representative subset of isolated SCVs is shown. (b,c) Isolated, gentamicin-resistant S. aureus SCVs from a single patient were co-cultured with ΔhemB (b) or ΔmenE (c) on solid media to assess for enhancement of growth. (d) Select gentamicin-resistant S. aureus were co-cultured with each other on solid media to demonstrate growth enhancement. A representative subset of growth-enhancing pairs is shown. Strain identities are as follows: VUCF001.1 = Staphylococcus epidermidis; VUCF001.2 and VUCF001.3 = Diphtheroid-like Gram-positive rods; VUCF002.1 = Streptococcus sobrinus; VUCF003.1 through VUCF003.5 = S. aureus.