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. 2014 Jun 3;76(9):1235–1240. doi: 10.1292/jvms.14-0209

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©2014 The Japanese Society of Veterinary Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.

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Fig. 4. — Expression and localization of canine SET isoforms. (A) HEK293T cells were transfected to express FLAGx3-tagged canine SET isoforms, and protein expression was analyzed by immunoblotting. VCP was used as a loading control. (B) The localization of endogenous SET in CMeC1 cells was examined by immunofluorescent staining using anti-SET (N-term) and anti-SET (Middle) (Green). Phalloidin (Red) and Hoechst (Blue) were used to stain F-actin and the nucleus. (C) HeLa cells were transfected to express FLAGx3-tagged canine SET isoforms and ZsGreen, and the localization of SET isoforms was determined by immunofluorescent staining using an anti-FLAG antibody (Red). Hoechst (Blue) was used to stain the nucleus.