Table 1. Primers used for PCR amplification.

Name	Primer sequences
SacI-cIL18 F	5′-GAGCTCATGGCTGCTAACCTAATAGAAG-3
SacI-cIL18 R	5′-GAGCTCATAGAACGGTTGGTCGGATG-3′
AflII-cIL12p40 F	5′-CCTTAAGATGCATCCTCAGCAGTTGGTCATCTCC-3′
KpnI-cIL12p40 R	5′-GGGTACCCTAACTGCAGGACACAGATGCCCAGTC-3′
NdeI-mature-cIL18 F	5′-GCATATGGTACTTTGGCAAGCTTGAACCTAAAC-3′
KpnI-mature-cIL18 R	5′-GGTACCCTAGCTCTTGTTTTGAACAGTGAAC-3′
NcoI-mature-cIL18 F	5′-CCATGGCCTACTTTGGCAAGCTTGAACCTAAAC-3′
BglII-mature-cIL18 R	5′-AGATCTCTAGCTCTTGTTTTGAACAGTGAAC-3′
NcoI-mature-mIL18 F	5′-ATAGCCATGGCTAACTTTGGCCGACTTCAC-3′
BglII-mature-mIL18 R	5′-GGGGAGATCTCTAACTTTGATGTAAGTTAGTG-3′
FseI-cIL18-CDV F	5′-GGCCGGCCaaactcattataaaaaacttagggctcaggtagtccaacaATGGCTGCTAACCTAATAGAAG-3′
FseI-cIL18-CDV R	5′-GGCCGGCCTCTACTAGCTCTTGTTTTGAACAGTG-3′
FseI-hIL2ss-CDV F	5′-GGCCGGCCTCTaaactcattataaaaaacttagggctcaggtagtccaacaATGTACAGGATGCAACTCCT-3′
FseI-hIL2ss-cIL18-CDV R	5′-GGCCGGCCCTAGCTCTTGTTTTGAACAGTGAAC-3′
FseI-hIL2ss-mIL18-CDV R	5′-GGCCGGCCCTAACTTTGATGTAAGTTAGTG-3′
CDV-N F	5′-TGGTTGGTGATCCGAAAATCAACGGACC-3′
CDV-N R	5′-CCCTCCCATGGAGTTTTCAAGTTCAACACC-3′

Restriction sites are underlined. CDV transcription signal unit sequences are described in lowercase letters.