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| Primary Screening | Secondary Screening | |||
|---|---|---|---|---|
| Mouse | No. of clone | α RBC clonea) | α RBC cloneb) | |
| (after re-cloning) | ||||
| B. rodhaini infected | A | 25 | 4 | 3 |
| B | 21 | 5 | 0 | |
| C | 15 | 4 | 2 | |
| Mock | A | 11 | 0 | 0 |
| B | 5 | 0 | 0 | |
| C | 4 | 0 | 0 | |
a) Total number of well showed hybridoma growth. All established clones were proceeded to ELISA test for detecting erythrocyte-reacting clones. b) Number of clone reacting with erythrocyte antigen.
