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Quantification of HBV ccc DNA copy numbers in HBsAg negative patients. (A) HBV ccc DNA was detected by PCR. The HBV plasmid of known concentration ranging from 102 to 107 copies/reaction was used for standard. (B) ccc DNA was quantified using real-time PCR. Standard curve of HBV ccc DNA was plotted by the threshold cycle (Ct). The ccc DNA copies of HBsAg loss patients were calculated by interpolation of standard curve.
