Table 1.
Comparison of BCL2 protein expression by three different antibodies in DLBCL.
| BCL2 Positivea n (%) |
P | |||
|---|---|---|---|---|
| 124 | E17 | SP66 | ||
| DLBCL, all subtypes (n=94) | 32 (34.0) | 58 (61.7) | 75 (79.8) | <0.0001 |
| ABC (n=36) | 13 (36.1) | 26 (72.2) | 32 (88.8) | <0.0001 |
| Non-GCBb (n=43) | 16 (37.2) | 33 (76.7) | 39 (90.7) | <0.0001 |
| GCB (n=38) | 14 (36.8) | 18 (47.4) | 27 (71.1) | 0.009 |
| BCL2gene status | ||||
| Translocation (+) (n=14) | 9 (64.3) | 11 (78.6) | 13 (92.9) | 0.183 |
| Amplification (+) (n=28) | 10 (35.7) | 22 (78.6) | 26 (92.9) | <0.0001 |
| Chrs. 18 polysomy (+) (n=6) | 1 (16.7) | 4 (66.7) | 4 (66.7) | 0.135 |
a
defined as ≥ 30% of the cells stained positive (medium to dark brown) in a 150 cell count
b
non-GCB subtype includes ABC and unclassifiable as determined by GEP and non-GCB as determined by IHC according to Hans classification (15).