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. 2014 Oct 8;3:588. doi: 10.1186/2193-1801-3-588

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© Zschemisch et al.; licensee Springer. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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Analysis of ESC line ES21. a. ES21 cells contributed to chimeric rats after blastocyst injection and b. showed germ line transmission. c. Metaphase spreading and giemsa staining proofed that ES21 cells at passage 12 were genetically stable containing a diploid set of chromosomes. d. Teratomas with tridermal tissues (white arrow: glandular endodermal structures; black star: muscle-like mesodermal tissue; black arrow: ectodermal neural tube formation) arose from ES21 cells after subcutaneous injection into immuno-deficient mice, H&E, (2.5×).